王宏伟 博士

教授
谈家桢生命科学创新奖获得者
科学探索奖获得者

主要科研领域与方向

利用冷冻电子显微学研究生物大分子复合体的结构与分子机理研究生物大分子复合体的结构与分子机理。

主要研究方向包括：

1. 更高效、更高分辨率的冷冻电镜方法学开发；

2. 核酸质量控制的分子机制和调控；

3. 细胞骨架和生物膜系统的协调机制。

代表性论文

1.Zi Yang, Jingjin Fan, Jia Wang, Xiao Fan, Zheng Ouyang*, Hong-Wei Wang*, Xiaoyu Zhou*. (2024) Electrospray-assisted cryo-EM sample preparation to mitigate interfacial effects. Nature Methods, 21(6):1023-1032.

2.Jie Xu, Xiaoyin Gao, Liming Zheng, Xia Jia, Kui Xu, Yuwei Ma, Xiaoding Wei, Nan Liu*, Hailin Peng, Hong-Wei Wang*. (2024) Graphene sandwich-based biological specimen preparation for cryo-EM analysis. Proc Natl Acad. Sci USA, 121(5):e2309384121.

3.Ju Zhou, Anhui Wang, Yinlong Song, Nan Liu, Jia Wang, Yan Li, Xin Liang, Guohui Li, Huiying Chu, Hong-Wei Wang*. (2023) Structural insights into the mechanism of GTP initiation of microtubule assembly. Nature Communications, 14(1):5980.

4.Xin You, Xing Zhang, Jing Cheng, Yanan Xiao, Jianfei Ma, Shan Sun, Xinzheng Zhang*, Hong-Wei Wang*, Sen-Fang Sui*. (2023) In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex. Nature, 616(7955):199-206.

5.Liming Zheng, Nan Liu, Xiaoyin Gao, Wenqing Zhu, Kun Liu, Cang Wu, Rui Yan, Jincan Zhang, Xin Gao, Yating Yao, Bing Deng, Jie Xu, Ye Lu,  Zhongmin Liu, Mengsen Li, Xiaoding Wei*, Hong-Wei Wang*, Hailin Peng*. (2022) Uniform thin ice on ultraflat graphene for high-resolution cryo-EM. Nature Methods, 20(1):123-130.

6.Ye Lu, Nan Liu, Yongbo Liu, Liming Zheng, Junhao Yang, Jia Wang, Xia Jia, Qinru Zi, Hailin Peng*, Yu Rao*, Hong-Wei Wang*. (2022) Functionalized graphene grids with various charges for single-particle cryo-EM. Nature Communications, 13(1):6718.

7.Shichen Su, Jia Wang, Ting Deng, Xun Yuan, Jinqiu He, Nan Liu, Xiaomin Li, Ying Huang, Hong-Wei Wang*, Jinbiao Ma*. (2022) Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD. Nature, 607(7918):399-406.

8.Xiao Fan, Jia Wang, Xing Zhang, Zi Yang, Jin-Can Zhang, Lingyun Zhao, Hai-Lin Peng, Jianlin Lei*, Hong-Wei Wang*. (2019) Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution. Nature Communications, 10(1):2386.

9.Nan Liu, Jincan Zhang, Yanan Chen, Chuan Liu, Xing Zhang, Kui Xu, Jie Wen, Zhipu Luo, Shulin Chen, Peng Gao, Kaicheng Jia, Zhongfan Liu, Hailin Peng*, Hong-Wei Wang*. (2019) Bioactive Functionalized Monolayer Graphene for High-Resolution Cryo-Electron Microscopy. Journal of the American Chemical Society, 141(9):4016-4025.

10. Zhongmin Liu, Jia Wang, Hang Cheng, Xin Ke, Lei Sun, Qiangfeng Cliff Zhang, Hong-Wei Wang*. (2018) Cryo-EM Structure of Human Dicer and Its Complexes with a Pre-miRNA Substrate. Cell, 173(5):1191-1203.