Jun-Jie Gogo Liu Ph.D.

Assistant Professor

Main Research Fields

The Liu lab uses multidisciplinary approaches to study the following topics:

(1)Development of gene-editing tools;

(2)Mechanism study about RNA involved nuclease machinery;(3)Functional and structural study of disease related LncRNA and the development of new therapy method.

Selected Publications

1. Tsuchida CA, Zhang SY, Doost MS, Zhao YQ, Wang J, O’Brien E, Fang H, Cheng-Ping Li, Li DY, Hai ZY, Chuck J, Brötzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA, Liu JJG*. Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity. Molecular Cell (2022).
2. Yang MY, Sun RR, Deng PJ, Yang YZ, Wang WJ, Liu JJG*, Chen CL*. Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search. Chem. Sci (2021). (Co-responding Author)
3. Liu JJ#,Wang HW#.（2020）Cryo-Electron Microscopy of Endogenous Yeast Exosomes. Methods Mol. Biol. , 2062:401-415. (Book Chapter, Corresponding Author)
4. Liu TY*, Liu JJ*, Aditham AJ, Nogales E, Doudna JA. Target preference of Type III A CRISPR-Cas complexes at the transcription bubble. Nature Communications,10(1), 3001(2019). (Co-first author)
5. Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knot GJ, Harrington L, Al-Shayeb B, Wanger A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA. CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature, 566,218-223 (2019).
6. Jiang F*, Liu JJ, Osuna BA*, Xu M, Berry JD, Rauch BJ, Nogales E, Denomy JB, Doudna JA. Temperature-responsive competitive inhibition of CRISPR-Cas9. Molecular Cell, 1016-016(2018). (Co-first author)
7. Wright AV*, Liu JJ*, Knott GJ, Doxzen KW, Nogales E, Doudna JA. Structures of the CRISPR genome integration complex. Science, 357, 1113-1118 (2017). (Co-first author)
8. Shing J*, Jiang F*, Liu JJ*, Bray NL, Rauch BJ, Baik SH, Nogales E, Bondy-Denomy J, Corn JE, Doudna JA. Disabling Cas9 by an anti-CRISPR DNA mimic. Science Advances, 3(7): e1701620(2017). (Co-first author)
9. Ma M*, Liu JJ*, Li Y, Huang Y, Ta N, Chen Y, Fu H, Ye MD, Ding Y, Huang W, Wang J, Dong MQ, Yu L, Wang HW. Cryo-EM structure and biochemical analysis reveal the basis of the functional difference between human PI3KC3-C1 and-C2. Cell Research, 27,989-1001(2017). (Co-first author)
10. Liu JJ, Niu CY, Wu Y, Tan D, Wang Y, Ye MD, Dong MQ, Huang N & Wang HW, CryoEM structure of yeast cytoplasmic exosome complex, Cell Research, 26, 822-837(2016).