联系方式

电话:010-62770270

邮箱:junjiegogoliu@tsinghua.edu.cn

刘俊杰 博士

助理教授

个人履历

2007-2011四川大学生命科学与技术基地班 学士

2011-2016北大-清华-NIBS联合培养(PTN)项目 博士

2016-2018劳伦斯-伯克利国家实验室生物物理和生物成像中心 博士后

2018-2020加州大学伯克利分校细胞分子生物学系 博士后

2018-2020美国生命科学研究基金(LSRF) 博士后研究员

2020.10- 至今 清华大学生命科学学院 助理教授

主要科研领域与方向

本实验室主要利用生物信息学,生物化学,生物物理学以及细胞生物学手段从事以下研究:(1)新型基因编辑工具开发;(2)与RNA相关的核酸酶机器工作机理研究;(3)重大疾病相关的lncRNA结构功能研究及潜在治疗手段开发。

代表性论文


1. Tsuchida CA, Zhang SY, Doost MS, Zhao YQ, Wang J, O’Brien E, Fang H, Cheng-Ping Li, Li DY, Hai ZY, Chuck J, Brötzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA, Liu JJG*. Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity. Molecular Cell (2022).

2. Yang MY, Sun RR, Deng PJ, Yang YZ, Wang WJ, Liu JJG*, Chen CL*. Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search. Chem. Sci (2021). (Co-responding Author)

3. Liu JJ#,Wang HW#.(2020)Cryo-Electron Microscopy of Endogenous Yeast Exosomes. Methods Mol. Biol. , 2062:401-415. (Book Chapter, Corresponding Author)

4. Liu TY*, Liu JJ*, Aditham AJ, Nogales E, Doudna JA. Target preference of Type III A CRISPR-Cas complexes at the transcription bubble. Nature Communications,10(1), 3001(2019). (Co-first author)

5. Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knot GJ, Harrington L, Al-Shayeb B, Wanger A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA. CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature, 566,218-223 (2019).

6. Jiang F*, Liu JJ, Osuna BA*, Xu M, Berry JD, Rauch BJ, Nogales E, Denomy JB, Doudna JA. Temperature-responsive competitive inhibition of CRISPR-Cas9. Molecular Cell, 1016-016(2018). (Co-first author)

7. Wright AV*, Liu JJ*, Knott GJ, Doxzen KW, Nogales E, Doudna JA. Structures of the CRISPR genome integration complex. Science, 357, 1113-1118 (2017). (Co-first author)

8. Shing J*, Jiang F*, Liu JJ*, Bray NL, Rauch BJ, Baik SH, Nogales E, Bondy-Denomy J, Corn JE, Doudna JA. Disabling Cas9 by an anti-CRISPR DNA mimic. Science Advances, 3(7): e1701620(2017). (Co-first author)

9. Ma M*, Liu JJ*, Li Y, Huang Y, Ta N, Chen Y, Fu H, Ye MD, Ding Y, Huang W, Wang J, Dong MQ, Yu L, Wang HW. Cryo-EM structure and biochemical analysis reveal the basis of the functional difference between human PI3KC3-C1 and-C2. Cell Research, 27,989-1001(2017). (Co-first author)

10. Liu JJ, Niu CY, Wu Y, Tan D, Wang Y, Ye MD, Dong MQ, Huang N & Wang HW, CryoEM structure of yeast cytoplasmic exosome complex, Cell Research, 26, 822-837(2016).