方显杨 博士

助理教授

主要科研领域与方向

本课题组聚焦于RNA整合结构生物学研究。我们一方面致力于发展RNA的体外与活细胞内位点特异性与区段选择性标记技术，发展多方法(包括小角X射线/中子散射技术、电子顺磁共振/核磁共振技术、X射线晶体学、单分子荧光共振能量转移、单分子纳米孔技术以及计算模拟)联用的RNA及其复合物高级结构研究的整合方案，另一方面致力于应用所发展的标记技术和结构研究整合方案，开展与人类疾病或重大生命过程密切相关的RNA(包括黄病毒，艾滋病毒与冠状病毒来源的病毒RNA，与致病细菌基因表达调控相关的核糖开关，参与转录后调控且与神经退行性疾病发生密切相关的mRNA元件)的高级结构、动态特性、相互作用与功能关系的研究，以期为发展RNA疗法提供依据。

代表性论文

1. Burkhard Endeward#, Yanping Hu#, Guangcan Bai, Guoquan Liu, Thomas F. Prisner*, Xianyang Fang*. Long-range distance determination in fully deuterated RNA with pulsed EPR spectroscopy. Biophysical Journal 2022，121(1):37-43.

2. Xiaolin Niu#, Ruirui Sun#, Zhifeng Chen, Yirong Yao, Xiaobing Zuo, Chunlai Chen*, Xianyang Fang*. Pseudoknot length modulates the folding, conformational dynamics and robustness of Xrn1 resistance of flaviviral xrRNAs. Nature Communications 2021, 12(1): 6417.

3. Junfeng Ma, Xiang Cheng, Zhonghe Xu, Yikan Zhang, Jaione Valle, Shilong Fan, Xiaobing Zuo, Iñigo Lasa, Xianyang Fang*. Structural mechanism for modulation of functional amyloid and biofilm formation by Staphylococcal Bap protein switch. The EMBO Journal 2021, 40(14): e107500. (Research Highlight on Nature Chemical Biology 2021, 17: 839)

4. Xiaolin Niu#, Qiuhan Liu#, Zhonghe Xu#, Zhifeng Chen, Linghui Xu, Lilei Xu, Jinghong Li*, Xianyang Fang*. Molecular mechanisms underlying the mechanical anisotropy of flaviviral exoribonuclease-resistant RNAs (xrRNAs). Nature Communications 2020, 11(1): 5496 (News and Views on Nature Chemical Biology 2021, 17: 933-934)

5. Yan Wang, Venkatesan Kathiresan, Yaoyi Chen, Yanping Hu, Wei Jiang, Guangcan Bai, Guoquan Liu, Peter Z. Qin*, Xianyang Fang.* Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions. Chemcial Science 2020, 11: 9655-9664.

6. Yan Wang, Yaoyi Chen, Yanping Hu, Xianyang Fang. Site-specific covalent labeling of large RNAs with nanoparticles empowered by expanded genetic alphabet transcription. Proceedings of the National Academy of Sciences USA 2020, 117(37): 22823-22832.

7. Yupeng Zhang#, Yikan Zhang#, Zhong-Yu Liu#，Meng-Li Cheng#, Junfeng Ma, Yan Wang, Cheng-Feng Qin*, Xianyang Fang*. Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Reports 2019, 20(11): e47016.

8. Xianyang Fang*, Malgorzata Michnicka, Yikan Zhang, Yun-Xing Wang, Edward P. Nikonowicz*. Capture and Release of tRNA by the T-Loop Receptor in the Function of the T-Box Riboswitch. Biochemistry 2017, 56(28): 3549-3558.

9. Xianyang Fang, Jason R. Stagno,Yuba Bh.andari,Xiaobing Zuo,Yun-Xing Wang. Small-angle X-ray scattering: a bridge between RNA secondary structures and three-dimensional topological structures. Current Opinion in Structural Biology 2015, 30: 147-160.

10. Xianyang Fang# , Jinbu Wang#, Ina P. O’Carroll#, Michelle Mitchell, Xiaobing Zuo, Yi Wang, Ping Yu, Yu Liu, Jason W. Rausch, Marzena A. Dyba, Jørgen Kjems, Charles D. Schwieters, Soenke Seifert, Randall E. Winans, Norman R. Watts, Stephen J. Stahl, Paul T. Wingfield, R. Andrew Byrd, Stuart F. J. Le Grice, Alan Rein*,Yun-Xing Wang*. An unusual topological structure of the HIV-1 rev response element, Cell  2013, 155(3): 594-605.

( #co-first author，*corresponding author)