联系方式

邮箱:chunlai@tsinghua.edu.cn

陈春来 博士

研究员

个人履历

1999-2003北京大学化学与分子工程学院 学士

2003-2008北京大学化学与分子工程学院 博士

2008-2015宾夕法尼亚大学 博士后

2015- 至今清华大学生命科学学院 研究员

主要科研领域与方向

发展单分子荧光方法和技术并用以揭示生物大分子的动态构象变化和分子机制

代表性论文

1. Yang M, Peng S, Sun R, Lin J, Wang N, Chen C*. (2018) Conformational dynamics of Cas9 governing DNA cleavage revealed by single-molecule FRET. Cell Rep, 22(2):372-382.

2. Peng S, Wang W * and Chen C*. (2018) Breaking the concentration barrier for single molecule fluorescence measurements. Chemistry, 24(5):1002-1009.

3. Peng S, Sun R, Wang W, Chen C*. (2017) Single-Molecule Photoactivation FRET: A General and Easy-to-Implement Approach to Break the Concentration Barrier. Angew. Chem. Int. Ed, 56(24):6882-6885.

4. Peng S, Yang M, Sun RN, Liu Y, Wang W, Xi Q, Gong H, Chen C*. (2017) Mechanism of actions of Oncocin, a proline-rich antimicrobial peptide, in early elongation revealed by single-molecule FRET. Protein & Cell., doi: 10.1007/s13238-017-0495-2.

5. Jamiolkowski RM, Chen C, Cooperman BS, Goldman YE. (2017) tRNA Fluctuations Observed on Stalled Ribosomes Are Suppressed during Ongoing Protein Synthesis. Biophys. J., 113(11):2326-2335.

6. Sun S, Li L, Yang F, Wang X, Fan F, Yang M, Chen C, Li X, Wang HW, Sui SF. (2017) Cryo-EM structures of the ATP-bound Vps4E233Q hexamer and its complex with Vta1 at near-atomic resolution. Nat. Conmun., 8:16064.

7. Chen C, Cui X, Beausang JF, Zhang H, Farrell I, Cooperman BS, Goldman YE. (2016) Elongation factor G initiates translocation through a power stroke. PANS, 113(27):7515-20.

8. Chen C, Zhang H, Broitman SL, Reiche M, Farrell I, Cooperman BS, Goldman YE. (2013) Dynamics of translation by single ribosomes through mRNA secondary structures. Nat. Struct. Mol. Biol., 20:582-588.

9. Chen C, Stevens B, Kaur J, Smilansky Z, Cooperman BS, Goldman YE. (2011) Allosteric vs. Spontaneous E-site tRNA Dissociation Early in Protein Synthesis. PNAS, 108:16980-16985.

10. Chen C, Stevens B, Kaur J, Cabral D, Liu H, Wang Y, Zhang H, Rosenblum G, Smilansky Z, Goldman YE, Cooperman BS. (2011) Single Molecule Fluorescence Measurements of Ribosomal Translocation Dynamics. Mol Cell, 42:367-377.