联系方式

电话:+86-10-62798651(实验室)

邮箱:hongweiwang@tsinghua.edu.cn

个人主页

王宏伟 博士

教授 中心执行主任

个人履历

1992-1996清华大学生物科学与技术系 学士

1996-2001清华大学生物科学与技术系 博士

2001-2006劳伦斯伯克利国家实验室生命科学部 博士后

2006-2008劳伦斯伯克利国家实验室生命科学部 研究科学家

2009-2011耶鲁大学分子生物物理与生物化学系 助理教授

2011- 至今清华大学生命科学学院 教授

主要科研领域与方向

我们利用冷冻电子显微学研究生物大分子复合体的结构与分子机理。主要研究方向包括:更高效、更高分辨率的冷冻电镜方法学开发;细胞骨架和生物膜系统的协调机制;核酸质量控制的分子机制和调控。

代表性论文

1. Mei K, Li Y, Wang S, Shao G, Wang J, Ding Y, Luo G, Yue P, Liu JJ, Wang X, Dong MQ, Wang HW*, Guo W*. (2018) Cryo-EM structure of the exocyst complex. Nat. Struct. Mol. Biol., 25:139-146

2. Fan X, Zhao L, Liu C, Zhang JC, Fan K, Yan X, Peng HL, Lei J*, Wang HW*. (2017) Near-Atomic Resolution Structure Determination in Over-Focus with Volta Phase Plate by Cs-Corrected Cryo-EM. Structure, 25:1623-1630

3. Xu J, Zhao L, Xu Y, Zhao W, Sung P*, Wang HW*. (2017) Cryo-EM structures of human RAD51 recombinase filaments during catalysis of DNA-strand exchange. Nat. Struct. Mol. Biol., 24:40-46

4. Liu JJ, Niu CY, Wu Y, Tan D, Wang Y, Ye MD, Liu Y, Zhao W, Zhou K, Liu QS, Dai J, Yang X, Dong MQ, Huang N, Wang HW*. (2016) CryoEM structure of yeast cytoplasmic exosome complex. Cell Research, 26:822-837

5. Qu G, Kaushal PS, Wang J, Shigematsu H, Piazza CL, Agrawal RK*, Belfort M*, Wang HW*. (2016) Structure of a group II intron in complex with its reverse transcriptase.Nat. Struct. Mol. Biol., 23:549-557

6. Song W, Wang J, Han Z, Zhang Y, Zhang H, Wang W, Chang J, Xia B, Fan S, Zhang D, Wang J*, Wang HW*, Chai J*. (2015) Structural basis for specific recognition of single-stranded RNA by Toll-like receptor 13. Nat. Struct. Mol. Biol., 22:782-787

7. Zhou Q, Huang X, Sun S, Li X, Wang HW*, Sui SF*. (2015) Cryo-EM structure of SNAP-SNARE assembly in 20S particle. Cell Research, 25:551-560

8. Chang S, Sun D, Liang H, Wang J, Li J, Guo L, Wang X, Guan C, Boruah BM, Yuan L, Feng F, Yang M, Wang L, Wang Y, Wojdyla J, Li L, Wang J, Wang M, Cheng G, Wang HW*, Liu Y*. (2015) Cryo-EM Structure of Influenza Virus RNA Polymerase Complex at 4.3 Å Resolution. Mol. Cell, 57:925-935

9. Liu JJ, Bratkowski MA, Liu X, Niu CY, Ke A*, Wang HW*. (2014) Visualization of distinct substrate-recruitment pathways in the yeast exosome by EM. Nat. Struct. Mol. Biol., 21:95-102

10. Taylor DW, Ma E, Shigematsu H, Cianfrocco MA, Noland CL, Nagayama K, Nogales E, Doudna JA*, Wang HW*. (2013) Substrate-specific structural rearrangements of human Dicer. Nat. Struct. Mol. Biol., 20:662-670